MS/MS-Based Bone Species Identification

PUSHH is a ETN focused in palaeoproteomics studies of human history. PUSHH has recruited 14 ESRs that will use palaeoproteomics to advance knowledge about biological and cultural evolution of humans. PUSHH brings together a multidisciplinary team of 10 beneficiaries and 8 partners from 10 different countries.

As part of PUSHH, this PhD project will apply data-independent acquisition (DIA) and data-dependent acquisition (DDA) tandem MS and ultra-fast peptide separation technology (~8 mins/run) developed by EvoSEP. The outcome will be to develop and apply a novel proteomic approach to identify the biological species of archaeological bone specimens by screening with Orbitrap technology. The advantages over peptide mass fingerprinting will be represented by the acquisition of a much richer dataset per sample, higher sensitivity, higher automation, and the ability to use off-the-shelf spectral identification software.

During this PhD project, the main goals are to acquire a deeper understanding of experimental proteomics techniques and to improve skills in computational analysis. Finally, this project is a great opportunity to collaborate and exchange opinions with different people, learn and discuss new ideas and methodologies in a multidisciplinary environment.

Read more about the PUSHH projects here!

This project has received funding from the European Union's EU Framework Programme for Research and Innovation Horizon 2020 under Grant Agreement No. 861389 - PUSHH